When produced in a sustainable and equitable manner, biofuels can increase Kenya’s energy self sufficiency, support rural development as well as reduce deforestation and GHG emissions compared to fossil fuels. The output of this study is intended to assist the national authorities of Kenya in better planning of the land use and assessment of the land resources and feedstocks suitable for production of biofuels (first generation). The study aimed at determining suitability areas of twelve biofuel feedstocks in Kenya and to identify high value biodiversity areas as well as areas of high socio-cultural values to be recommended for exclusion in biofuel production. The overarching goal of the study is to ensure that, when properly implemented, biofuel development will be economically productive alongside equitable land use, environmental preservation and reliable food production.