UC San Diego researchers have developed a ligand sensing fluorescent enzyme assay for detecting quantifying and evaluating hazardous organophosphate pesticide and nerve agent exposure. The technology utilizes fluorescently labeled mutants of the acetylcholinesterase enzyme (AChE) that exhibit fluoresence wavelength shifts upon ligand binding.
Benefits
The assay does not require reagent addition can distinguish between organophosphates and can be used in conjunction with laser microarray and capillary electrophoretic techniques for rapid detection of organophosphate conjugated AChE.
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