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Bacillus amyloliquefaciens KJ-6 and the method for decomposing to citrus tree sawdust using it


1. Separation of B. amyloliquefaciens KJ-6. - Identification by 16S rDNA base sequencing analysis - Method of culture of B. amyloliquefaciens KJ-6 - Production of B. amyloliquefaciens KJ-6 2. Evaluation of enzyme hydrolytic activities - Measurement of cellulolytic activity - Measurement of cellulase activitiy - Measurement of peroxydase activity - Measurement of amylase activity 3. Evaluation of anti-microbial activity The present invention relates to enzymatic hydrolysis activities antibacterial activity and the cultivation of Bacillus amyloliquefaciens (B. amyloliquefaciens) KJ- 6 (KACC 91260P) derived the citrus tree and its by-product. In accordance with the presesent invention new bacterial strains of the species B. amyloliquefaciens KJ- 6 is showed results the high stability celluloytic activity and anti-microbial activitiy.


Agricultural waste generated in field and processing sites is generally discarded without being further used. This waste can be handled in several ways including direct combustion landfill and composting. Composting is an effective organic matter degrading process when the appropriate conditions for microbial activity are given. This present invention is able to be affected the degrading agricultural waste by enzymatic hydrolysis of B. amyloliquefaciens KJ-6 derived from citrus tree sawdust. This microbial is derived from citrus tree Bacillus amyloliquefaciens KJ-6 is - To improve hydrolysis the cellulose in soil - To improve high CMCase against phytopathogenic microorganisms - Excellent antimicrobial activities - To enable utilizing fermentation products; .

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