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In-situ transestrification is a method by which cell membranes disintegrate by disrupting the phospholipid bilayer structure of biomembrane. The reaction transforms fatty acids found in wastes to methyl esters a valuable feed used in the production of high-value biodiesel fuels. Additionally the extraction of fatty acids in cell membranes results in the solubilization of cell contents due to disintegration of the membrane. Recycling this solubilized sludge back to an aeration tank will reduce the net sludge production.
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